تأثیرپوشش‎دار و پلت کردن بذر با استفاده از باکتری‎های محرک رشد بر جوانه‌زنی و رشد گیاهچه چغندرقند

نوع مقاله: کامل علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد تکنولوژی بذر دانشگاه شاهد

2 استادیار گروه زراعت و اصلاح نباتات دانشگاه شاهد

3 دانشیار موسسه تحقیقات چغندرقند

4 دانشیار موسسه تحقیقات آب و خاک

چکیده

در این تحقیقتاثیر دو روش پوشش­دار و پلتکردن یک رقم بذر چغندرقند در12 سطح باکتری (شاهد، کاربرد انفرادی باکتری‌های ازتوباکتر، باسیلوس، سودوموناس، آزوسپیریلوم، تلفیق دوتایی و تلفیق مجموع باکتری­ها با هم با غلظت 1-CFU ml 107 (تعداد کلونی تشکیل شده باکتری در میلی لیتر) بر درصد و سرعت ظهور گیاهچه، طول و وزن ریشه چه و ساقه چه، تعداد گیاهچه های عادیوغیر عادی، شاخص طولی و وزنی بنیه بذر و ماندگاری جمعیت باکتری ها در پوشش بذر پس از شش ماه پس از تیمار کردن و نگهداری بررسی شد. آزمایش به صورت فاکتوریل دو عاملی در قالب طرح بلوک­های کامل تصادفی با چهار تکرار درسال 1393 انجام شد. کاربرد باکتری بر صفات وزن تر گیاهچه، درصد گیاهچه عادی، شاخص وزنی و طولی بنیه بذر اثر معنی دار گذاشت ولی دو روش پوشش و پلت کردن بذر بر هیچ یک از صفات معنی دار نبود. نتایج نشان داد که پوشش بذر با باکتری سودوموناس موجب افزایش  160، 50، 128 و 90 درصدی نسبت به تیمار شاهد به ترتیب در وزن ترگیاهچه، درصدگیاهچه عادی شاخص طولی بنیه بذر و شاخص وزنی بنیه بذر نسبت به تیمار شاهد شد. همچنین تلفیق باکتری سودوموناس و ازتوباکتر نیز مناسب بود. نتایج قدرت ماندگاری باکتری پس از شش ماه در بذرهای پلت شده در مقایسه با بذرهای پوشش دار شده بیشتر بود. جمعیت باکتری های باسیلوس، سودوموناس، آزوسپیریلوم و ازتوباکتر در روی بذرهای پوشش دار شده به ترتیب 25000، 15950، صفر و 14100 CFU بود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of seed coating and pelleting with plant growth promoting rihzobacteria on germination and seedling growth of sugar beet (Beta vulgaris)

نویسندگان [English]

  • H. Mahdavi 1
  • S. Maleki Farahani 2
  • M.A. Chegini 3
  • H. Besharati 4
1 Msc Student of University of Shahed, Iran
2 Assistant professor of University of Shahed, Iran
3 Associate Professor of Sugar Beet Seed Institute (SBSI)- Agricultural Research Education and Extension Organization (AREEO), Iran
4 Associate Professor of Soil and Water Institute- , Iran
چکیده [English]

In this study, the efficiency of two seed coating methods including coating and pelleting and 12 bacteria levels including control, Azotobacter, Bacillus, Pseudomonas, Azospirillum, combination of two bacteria, and combination of all bacteria with 1-CFU ml 107 concentration was evaluated on germination, seedling growth, and bacterial survival on seed coat after 6 months in sugar beet cultivar Pars. The experiment was conducted in a factorial arrangement based on a randomized complete block design with four replications in 2015. Results showed significant effect of bacteria on seedling fresh weight, normal seedling percentage, and seed vigor; however, the two coating methods had no significant effect on the above-mentioned traits. Seed coating with Pseudomonas resulted in 160, 50, 128 and 90% increase in seedling fresh weigh, normal seedling percentage, and seed vigor compared with control. The effect of Pseudomonas and Azotobacter combination was also positive. Bacterial survival after 6 months was higher in pelleted seeds than coated seeds. The Bacillus, Pseudomonas, Azospirillum and Azotobacter population concentration on coated seeds were 25000, 15950, 0 and 14100 CFU, respectively.

کلیدواژه‌ها [English]

  • germination
  • Growth promoting bacteria
  • seed
  • Seed coating and pelleting
  • sugar beet
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