باکتری‌های همراه بذر چغندرقند: شناسایی و اثر آن‌ها بر جوانه‌زنی بذر

نوع مقاله: کامل علمی - پژوهشی

نویسندگان

1 گروه گیاهپزشکی دانشگاه بوعلی سینا همدان

2 همدان گروه گیاهپزشکی دانشگاه بوعلی سینا

چکیده

بذر چغندرقند دارای اهمیت ویژه‌ای است زیرا به‌عنوان انتقال‌دهنده باکتری‌های مفید و مضر نقش ایفا می‌کند. جهت شناسایی و تعیین اثر باکتری­های همراه بذر چغندرقند بر جوانه­زنی، بذر بوجاری­شده و ضد­عفونی نشده از بخش تحقیقات چغندرقند مرکز تحقیقات کشاورزی استان همدان تهیه گردید. درمجموع تعداد 80 استرین اندوفیت و اپی­فیت از بذر چغندرقند جداسازی شد. بر پایه الگوی پروتئینی به روش لاملی، تعداد سه الکتروتیپ نماینده انتخاب‌شده و خصوصیات بیوشیمیایی و مولکولی آن‌ها بررسی گردید. بر اساس نتایج آزمون‌های بیوشیمیایی و تعیین توالی ژن 16S rRNA استرین­های نماینده، گونه‌های Stenotrophomonas rhizophila و Pseudomonas geniculata به‌عنوان گونه‌های اپی­فیت و گونه Stenotrophomonas maltophilia به‌عنوان تنها گونه اندوفیت بذر چغندرقند شناسایی شدند. جهت انجام آزمون جوانه‌زنی بذر، سه گروه متفاوت ازلحاظ تأثیر استرین­ها بر جوانه‌زنی بذور چغندرقند ایجاد گردید. با تلقیح پنج استرین نماینده از هر گروه در قالب طرح کاملاً تصادفی با چهار تکرار و هر تکرار شامل یک پتری دیش با 10 عدد بذر، آزمون جوانه‌زنی بذر انجام شد. بر اساس نتایج، بذور تلقیح شده با استرین­های نماینده S. maltophilia، بیشترین میزان درصد جوانه­زنی (90 درصد)، سرعت جوانه‌زنی (0/0174 بر ساعت)، وزن‌تر ساقه­چه (100 میلی­گرم) و ریشه‌چه (126/2 میلی‌گرم)، وزن خشک ساقه­چه (50 میلی‌گرم) و ریشه‌چه (43/7 میلی‌گرم)، طول ساقه­چه (6/5 سانتی‌متر) و ریشه‌چه (8 سانتی‌متر) و کم‌ترین میزان یکنواختی بذر (138/7 ساعت) را نسبت به شاهد دارا بودند. بنابراین می‌توان پیشنهاد کرد که به‌منظور بهبود شاخص‌های جوانه‌زنی و رشد گیاهچه تلقیح بذور چغندرقند با باکتری S. maltophilia صورت گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

Sugar beet seed-associated bacteria: their identification and effects on seed germination

نویسندگان [English]

  • Milad Aeini 1
  • Gholam Khodakaramian 2
چکیده [English]

Sugar beet seed plays an important role in the transmission of beneficial or detrimental bacteria. To identify and evaluate the effects of sugar beet seed-associated bacteria on seed germination, cleaned but not disinfected seeds were provided from Sugar Beet Research Department at Hamadan Agricultural Research Center. A total of 80 endophyte and epiphyte bacterial strains were isolated from the seeds. Based on the protein pattern and according to Laemmli method, three representative electrotypes were selected and their biochemical and molecular characteristics were evaluated. Based on the biochemical test results and sequencing of the 16S rRNA gene of the representative strains Stenotrophomonas rhizophila and Pseudomonas geniculata were identifiedas epiphytic and Stenotrophomonas maltophilia as endophytic species, respectively. To perform seed germination test, three different groups were generated based on the strain effect on seed germination. Seed germination test was conducted based on the inoculation of five representative strains from each group in completely randomized design with four replications. Each replication included a Petri dish containing 10 seeds. Results showed that seeds inoculated by S. maltophilia strain had the highest germination percentage (90), germination rate (0.0174 per hour), shoot fresh weight (100 mg), radicle fresh weight (126.2 mg), shoot dry weight (50 mg), radicle dry weight (43.7 mg), shoot length (6.5 cm), radicle length (8 cm), and the lowest seed uniformity compared with control. In order to improve germination indices and seedling growth, it is recommended to inoculate sugar beet seed with S. maltophilia bacterium.

کلیدواژه‌ها [English]

  • Endophyte
  • epiphyte
  • Seed germination
  • sugar beet
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