شیوع گسترده تیپ A ویروس زردی نکروتیک رگبرگ چغندرقند در ایران

نوع مقاله: کامل علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری گروه بیماری شناسی گیاهی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 دانشیار موسسه تحقیقات اصلاح و تهیه بذر چغندرقند، سازمان تحقیقات آموزش و ترویج کشاورزی ، کرج، ایران

3 استادیار گروه بیماری شناسی گیاهی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

4 استادیار پژوهش، موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج

5 دانشیار موسسه تحقیقات اصلاح و تهیه بذر چغندرقند، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

هدف از این تحقیق، بررسی ژنوتیپ­های مختلف Beet necrotic yellow vein (BNYVV) در ایستگاه­های تحقیقاتی چغندرقند کشور شامل طرق در خراسان رضوی، زرقان در فارس، میاندوآب در آذربایجان غربی و اکباتان در همدان بود. هم­زمان نمونه­هایی از مزارع حوزه کارخانه­های قند تربت­جام در خراسان رضوی، نهاوند در استان همدان، کمال­آباد در استان البرز، نقده در استان آذربایجان­غربی و قزوین در استان قزوین و نیز مزارع کشاورزان اراک در استان مرکزی مطالعه شد. جمعا 58 نمونه آلوده ریشه چغندرقند از این ایستگاه­ها و مزارع فوق مورد بررسی قرار گرفتند. همچنین تعداد 30 نمونه خاک آلوده از مزارع چغندرقند مغان، طرق، زرقان و کمال­آباد کرج جمع آوری و جهت تعیین نوع تیپ ویروس عامل بیماری رقم حساس در آنها کشت شد. برای تعیین نوع تیپ­های A و B، ناحیه TGB ژنوم ویروس BNYVV با روش multiplex PCR تکثیر شد. به علاوه، با تکثیر قسمتی از ژنوم RNA5  ویروس از طریق تکنیک مولکولی RT-PCR و جفت آغازگر اختصاصی، ردیابی تیپ  P یا J انجام گردید. بر این اساس در بین نمونه­های آلوده مورد بررسی با روش multiplex PCR و جفت آغازگرهای اختصاصی A و B تنها قطعه 324 جفت بازی تکثیر گردید. به این ترتیب در بین 88 نمونه­ آلوده مورد بررسی، بدون استثنا تیپ A شناسایی و هیچ­کدام از تیپ­های B، P و  Jردیابی نشدند. جدایه­های BNYVV حاوی RNA5 در هیچ­یک از نمونه ها یافت نشد.

کلیدواژه‌ها


عنوان مقاله [English]

Extensive outbreak of the type A of Beet Necrotic Yellow Vein Virus in Iran

نویسندگان [English]

  • M. Ebrahim-Ghomi 1
  • B. Mahmoodi 2
  • F. akhshanderoo 3
  • M. Naderpour 4
  • P. Norouzi 5
1 PHD Student of Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad university, Tehran, Iran
2 Associate professor of Sugar Beet Seed Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3 Assistant professor of Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad university, Tehran, Iran
4 Assistant professor of Seed and Plant Certification and Registration Institute(SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
5 Associate professor of Sugar Beet Seed Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

The present study aimed to evaluate different Beet necrotic yellow vein virus (BNYVV) genotypes in sugar beet research stations in Iran including Toroq, Zarqan, Miandoab and Ekbatan. Some samples were also collected  from sugar factories’ field in Torbat-e Jam, Nahavand, Kamalabad, Naqadeh and Qazvin and also sugar beet fields in Arak. A total of 58 infected sugar beet root samples were collected. In addition, 30 soil samples were collected from sugar beet fields in Moghan, Toroq, Zarqan and Kamalabad, and a susceptible cultivar was planted in them to determine the causal agent type. To detect type A or B of the virus, TGB region of the virus genome was multiplied using multiplex-PCR. Moreover, partial multiplication of  RNA5 using RT-PCR and specific primers was performed to detect type P or J. Only 324 bp fragment was multiplied using multiplex-PCR and specific primers designed for type A or B. Therefore, among 88 samples, only type A was detected. No BNYVV isolates containing RNA5 was observed in any of the samples.

کلیدواژه‌ها [English]

  • BNYVV
  • Multiplex PCR
  • Rhizomania
  • RNA5
  • sugar beet
  • Type A
 

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