سطوح بهینه زادمایه جهت ارزیابی مقاومت به نماتد مولد گره ریشه چغندرقند در شرایط گلخانه

نوع مقاله: کامل علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران

2 دانشیار مؤسسه تحقیقات چغندرقند

3 استاد گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس

4 دانشیار گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس،

چکیده

چغندرقند یکی از گیاهان میزبان گونه­های مختلف نماتد مولد گره ریشه است. در این مطالعه، ابتدا تأثیر شش سطح زادمایه 250+250، 500، 500+250، 750، 500+500 و تعداد 1000 لارو سن دوم Meloidogyne javanica در 450 سانتی­متر مکعب خاک، روی رقم حساس جلگه بررسی شد. نتایج نشان داد که تمامی سطوح زادمایه قادر به آلوده کردن بوته­های چغندرقند بودند و از نظر تعداد گره­های حاصل از مایه­زنی طی یک یا دو نوبت، اختلاف آماری وجود نداشت. بنابراین، 500 لارو سن دوم نماتد مولد گره ریشه کمترین سطح زادمایه است که قادر به آلوده کردن چغندرقند در شرایط گلخانه می­باشد. در آزمایش دوم تیمار مطلوب، بر روی دو رقم جلگه (حساس به نماتد مولد گره ریشه) و پائولتا (رقم تجاری مقاوم به نماتد سیستی چغندرقند) و هفت خانواده نیمه­خواهری که از یک جمعیت (SB33) مقاوم به نماتد مولد گره ریشه منشأ گرفته بودند مایه­زنی شدند تا عمومی بودن تیمار مناسب غربال­گری، بررسی شود. تعداد گره­های تشکیل شده روی ریشه ملاک ارزیابی مقاومت بود. بر اساس نتایح حاصله رقم جلگه با میانگین گره­ بالای 100 عدد جزو گیاهان حساس و هفت خانواده نیمه­خواهری جمعیت SB33 با میانگین تعداد گره­ کمتر از یک، جزو گیاهان مقاوم دسته­بندی شدند. رقم تجارتی پائولتا که به نماتد سیستی چغندرقند مقاوم بود به نماتد مولد گره ریشه حساسیت بالایی داشت.

کلیدواژه‌ها


عنوان مقاله [English]

Optimal inoculum levels for the resistance screening of sugar beet to root- knot nematode under greenhouse condition

نویسندگان [English]

  • M. Bakooie 1
  • S.B. Mahmoudi 2
  • E. Pourjam 3
  • N. Safaie 4
1 Ph.D Candidate of Dep. Plant Pathology, College of Agriculture, Tarbiat Modares University, Iran
2 Associate professor of Sugar Beet Seed Institute (SBSI), Iran
3 Professor of Dep. Plant Pathology, College of Agriculture, Tarbiat Modares University, Iran.
4 Associate professor of Dep. Plant Pathology, College of Agriculture, Tarbiat Modares University, Iran.
چکیده [English]

Sugar beet (Beta vulgaris) is one of the hosts for different root-knot nematode species. In this study, firstly the effect of different inoculum levels including 250+250, 500, 500+250, 750, 500+500 and 1000 second stage juveniles of Meloidogyne javanica were evaluated on a susceptible variety named Jolgeh. The results showed that all inoculum levels were suitable for infection on sugar beet plants and that the gall numbers at one and two time inoculations were not statistically different (p≥0.01). Therefore, 500 second stage juvenile of root-knot nematode was the lowest inoculum level that could be used to infect sugar beet plants in the greenhouse. In the second experiment, the best treatment was applied on Jolgeh (susceptible to root-knot nematode) and Pauletta (commercial resistant variety to cyst nematode) varieties and also seven half-sib families derived from SB33 population (carrying root-knot nematode resistance gene), to evaluate its generalization for screening of resistant/susceptible genotypes. The total number of root galls was taken as nematode resistance criterion. Results showed that Jolgeh and Pauletta varieties with average number of more than 100 galls were considered as highly susceptible varieties whereas the seven half-sib families with average number of 10 galls (<10)were classified as resistant varieties.

کلیدواژه‌ها [English]

  • Optimal inoculum level
  • resistance
  • Root-knot nematode
  • sugar beet
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