تغییرات فیتوشیمایی گیاه چغندر (Beta vulgaris L)در پاسخ به پرتو ماوراءبنفش B

نوع مقاله: کامل علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری تخصصی زیست‌شناسی-فیزیولوژی گیاهی، گروه زیست‌شناسی، دانشکده علوم، دانشگاه محقق اردبیلی، اردبیل، ایران.

2 دانشیار گروه زیست‌شناسی، دانشکده علوم، دانشگاه محقق اردبیلی، اردبیل، ایران.

چکیده

موجودات زنده واجد سازوکارهایی برای رویارویی با اثرات مضر پرتو فرابنفش خورشید می­باشند. در این آزمایش، آثار سه دوز پرتو فرابنفشB بر روی برخی ویژگی­های فیزیولوژیک رقم BR1 گیاه چغندرقند (Beta vulgaris L) درقالب طرح کاملاً تصادفی درسه تکرار مورد بررسی قرار گرفت. تمامی گیاهان در اتاقک رشد با دمای ۲۰/۲۵ درجه سانتیگراد (شب/روز) با دوره روشنایی/ تاریکی ۱۶ ساعت و هشت ساعت، به مدت 30 روز رشد داده شدند. سپس گیاهان تحت چهار تیمار شاهد و سه تیمار ۰۴۲/۳، ۰۸۴/۶ و ۱۲۶/۹ کیلو ژول بر متر مربع در روز پرتو فرابنفش B به مدت یک هفته قرار داده شدند. ببیشترین میزان پرتو فرابنفش (۱۲۶/۹ کیلو ژول بر متر مربع در روز) موجب کاهش ۱۱ درصدی میزان قندهای محلول بافت برگی گیاه شد. همچنین دوزهای ۰۴۲/۳، ۰۸۴/۶ و ۱۲۶/۹ کیلو ژول بر متر مربع در روز پرتو فرابنفش B موجب افزایش ۸۰، ۸۲ و ۸۶ درصدی فلاوونوئیدها گردیده است. بالاترین میزان پرتو فرا بنفش سبب افزایش ۲۴ درصدی ترکیبات فنلی کل در گیاه چغندرقند شد. پرتو فرا بنفش B موجب افزایش معنی­دار مقادیر بتالائین­ها (بتانین و بتاگزانتین) نیز گردید. فعالیت آنتی­اکسیدانتی عصاره متانولی برگ­های چغندرقند تحت پرتو فرا بنفش B افزایش معنی­داری داشت. بررسی فعالیت آنتی­اکسیدانتی بتالایین ها نشان داد که میزان فعالیت آنتی اکسیدانتی بتاگزانتین بیشتر از بتانین می باشد. افزایش شدت پرتو فرابنفش B موجب بالا رفتن میزان متابولیت های ثانویه چغندر قند و در نتیجه منجر به ارتقای توان آنتی اکسیدانی این گیاه گردید.

کلیدواژه‌ها


عنوان مقاله [English]

Phytochemical variations in sugar beet (Beta vulgaris L) in response to Ultraviolet-B radiation

نویسندگان [English]

  • P. Rahimzadeh 1
  • S.M. Razavi 2
1 Ph.D student, Department of Biology, University of Mohaghegh Ardabili, Ardabil, Iran.
2 Associate Professor, Department of Biology, University of Mohaghegh Ardabili, Ardabil, Iran.
چکیده [English]

All living organisms have mechanisms to deal with the harmful effects of ultraviolet (UV) radiation. In this study, the effects of three UV-B doses on some physiological properties of sugar beet cultivar, BR1 was evaluated in a completely randomized design with three replications. All plants were kept in a growth chamber at 25/20 ºC (day/night) with a 16 hours light and 8hours dark photoperiod for 30days. Then, plants were divided into 4 groups including control and three experimental groups exposed to 3.042, 6.084 and 9.126 KJm-2d-1UV-B radiation, respectively for one week. Results indicated that the highest amount of UV-B (9.126 KJm-2d-1) induced an 11% reduction in soluble sugar content of the leaf tissue. Treatment with 3.042, 6.084 and 9.126 KJm-2d-1UV-B resulted in 80, 82, and 86% increase in flavonoids content, respectively. Moreover, the highest amount of UV-B caused a 24% increase in total phenolic compounds content. Furthermore, UV-B radiation caused a significant increase in amounts of betalains (betanin and betaxanthin). The antioxidant activity of leaf methanolic extract increased under UV-B radiation. Evaluation of betaninand betaxanthin antioxidant activity also revealed that these pigments cause α, α-diphenyl-β-picrylhydrazyl free radical inhibition and betaxanthinhasa higher antioxidant activity than betanin. Increase in UV irradiation intensity increased the secondary metabolites and, consequently, increased the antioxidant capacity of the crop.

کلیدواژه‌ها [English]

  • Sugarbeet
  • Ultraviolet B
  • Betalain
  • chromatography
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